Thp1-luciatm isg cells
WebTo cite this cell line use: THP1-Lucia ISG (RRID:CVCL_X587) Population: Japanese. Characteristics: The Lucia luciferase reporter gene is under the control of an ISG54 … Webstimulated response elements. As a result, THP1-Blue™ ISG cells allow the monitoring of IRF activation by determining the activity of SEAP. The levels of IRF-induced SEAP in the …
Thp1-luciatm isg cells
Did you know?
WebCentrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of ... WebFeb 26, 2024 · Microarray analysis, qRT-PCR and flow cytometry were used to analyse TLR7/8 signalling pathways in monocytes and macrophages after stimulation with agonists. Our data indicated that TLR8 agonists activated the NF-κB- and IRF-mediated pathways in THP-1 cells, whereas TLR7 agonists did not. However, silent TLR8 and enhanced TLR7 …
WebFigure S5. Endogenously produced LL-37 facilitates the cGAMP-mediated host defense against HSV-1. Related to Figure 5. (A) WT and STINGCRISPR-/- THP1-Lucia ISG cells were infected with or without HSV-1 (MOI=1) for 24 h and … WebRAW-Lucia™ ISG cells were generated from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible Lucia luciferase …
WebIn general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: [1] Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglobulins. Production of IL-1. WebThe THP1-Lucia ISG cells and RAW-Lucia ISG cells were stimulated with cGAMP (1–3 μM) and/or increasing concentrations (1, 2, 5, 10, 20, 40 μg/mL) of LL-37, or a combination of cGAMP (1–3 μM) and PFO (0.01–1 μg/mL) for 12–24 h and the ISRE reporter activity was determined following the standard protocol (Invitrogen). The ...
WebJournal: Journal of Virology Article Title: HIV-1 Activation of Innate Immunity Depends Strongly on the Intracellular Level of TREX1 and Sensing of Incomplete Reverse Transcription Products doi: 10.1128/JVI.00001-18 Figure Lengend Snippet: THP-1HI and THP-1KO cell lines. TREX1 overexpression and CRISPR/Cas9-mediated targeting of …
WebMethods We collected sera from patients with SLE (n=64), patients with other autoimmune diseases (n=31) and healthy controls (n=35), and assayed them using a cell-based reporter system that enables highly sensitive detection of IFN-I and ISG-inducing activity. We used Toll-like receptor-specific reporter cells and reporter cells harbouring knockouts of cGAS, … cota stopsWebplate (1 x 105 cells/well). 2. Treat THP1-Lucia™ ISG cells with 20 PMA (final μl of concentration 20-50 ng/ml) for 3 hours at 37°C in 5% CO2. 3. Wash cells gently with pre … maestro necesito un milagro cancionWebApr 15, 2024 · CODEHEK293 (human)HEK-Blue™ ISGInterferon Regulatory Factor (IRF)-Inducible cells hkb-isg-1HEK-Blue™ IL-6Human IL-6 Reporter Cells hkb-hil6HEK-Blue™ IL-1β CellsHuman and murine interleukin-1β reporter cells hkb-il1bv2HEK-Blue™ TNFα CellHuman TNF-α Reporter Cells hkb-tnfdmyd※ Anti-PD-1/PD-L1 Cell-based Assay : … cotasulWebThe NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring NF-κB (nuclear factor κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or NOD agonists, endogenous NF-κB ... cotass marineWeb• The functionality of THP1-HMGB1-Lucia™ cells was tested using inflammasome inducers, such as nigericin. • The stability of this cell line for 20 passages following thawing has … cota street corona caWebAug 7, 2024 · I recently started working with THP-1 cells as an alternative to MDMs, and have been having difficulty growing my culture. We are trying to get them to a point where I can try a couple of ... maestro muten dragon ballWebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … maestro musica